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Tissue Freezing

What is Tissue Freezing?

Tissue freezing, also known as cryopreservation, is a medical procedure that involves preserving biological tissues, such as embryos, eggs, sperm, and other body tissues, at very low temperatures. By freezing these biological materials, they can be stored indefinitely, maintaining their viability and integrity until they are required for future use, whether for medical treatments, research, or fertility preservation.

Tissue Freezing

Types of Tissue Freezing

  • Embryo Freezing: Preserves embryos for future use in assisted reproductive technology (ART) treatments.
  • Egg Freezing: Helps women preserve their fertility by storing mature eggs for future conception.
  • Sperm Freezing: Allows men to store sperm for various reasons, including medical treatments that may affect fertility.
  • Tissue Freezing for research: Some body tissues, such as ovarian or testicular tissue, are frozen to contribute to research and advancements in regenerative medicine.

Benefits of Tissue Freezing

  • Preservation of biological materials: Tissue freezing allows for the long-term storage of biological materials, ensuring their viability and quality for future use.
  • Fertility preservation: Individuals facing medical treatments that could impact fertility, such as chemotherapy or radiation, can benefit from tissue freezing to preserve their reproductive potential.
  • Medical research: The ability to preserve biological materials contributes to advancements in medical research and regenerative medicine.
  • Treatment planning: Tissue freezing offers flexibility in scheduling medical treatments and procedures, allowing for better planning.

How Tissue Freezing is performed?

Consultation and Assessment:

  • Step 1: Before the procedure, a detailed consultation takes place where medical professionals evaluate the patient’s medical history, reproductive goals, and overall health.
  • Step 2: During this assessment, patients can discuss their concerns and expectations, and the medical team provides guidance on the appropriate steps and preparation required.

Preparation and Collection of Biological Material:

  • Step 1: The preparation process may involve ovarian stimulation, hormone treatments, or other medications to optimize the quality and quantity of biological material.
  • Step 2: Once ready, the biological material such as eggs, sperm, or embryos is collected under controlled conditions. For instance, for egg collection, a minor surgical procedure called follicular aspiration may be performed. During follicular aspiration, a fine needle is inserted into the ovaries to extract mature eggs from the ovarian follicles.
  • Step 3: For sperm collection, a sample may be provided through a sperm analysis, often collected through masturbation in a sterile environment.

Processing and Cryopreservation:

  • Step 1: After collection, the biological material undergoes processing to remove any fluids or elements that could affect the quality of cryopreservation.
  • Step 2: The material is then exposed to a cryoprotectant solution, which helps protect the cells from damage during the freezing process.
  • Step 3: Next, the biological material is gradually cooled using controlled-rate freezing technology. This process prevents the formation of ice crystals, which can damage cell structures during freezing.
  • Step 4: The material is then plunged into liquid nitrogen, typically at a temperature of -196°C, where it remains stored in specialized cryogenic storage tanks.


  • Step 1: The preserved biological material is stored in specialized storage containers, such as cryovials or straws, in nitrogen tanks at extremely low temperatures.
  • Step 2: These storage systems are designed to maintain a stable and controlled environment to ensure the biological material remains viable and unaffected by external temperature fluctuations.

Post-Procedure Care and Monitoring:

  • Step 1: Patients receive post-procedure care and support, including guidance on recovery and any necessary follow-up consultations.
  • Step 2: The medical team monitors the quality and stability of the preserved biological material to ensure its integrity over time.

By understanding each step of the egg freezing process, patients can approach their fertility treatment with confidence, knowing that they are in capable hands at Shukan Hospital & IVF Centre.

Risks of Tissue Freezing

  • Technical risks: There may be slight technical risks associated with cryopreservation procedures, including the potential for minor damage to biological materials during freezing or thawing.
  • Emotional and Psychological factors: Some individuals may experience emotional and psychological stress during the process, particularly in relation to fertility preservation.

At Shukan Hospital, we minimize these risks through meticulous planning, advanced technology, and comprehensive pre-operative and post-operative care.

Why choose Shukan Hospital & IVF Centre in Ahmedabad for Tissue Freezing?

  • We have inhouse team, experience embryologists and andrologists full time.
  • Our medical professionals provide comprehensive care and personalized support throughout the tissue freezing process.
  • Our team of experienced medical professionals is highly skilled in advanced cryopreservation techniques, ensuring optimal results and patient satisfaction.
  • We utilize the latest cryopreservation technologies to ensure the highest quality and preservation standards for biological materials.
  • Each consultation is personalized to meet individual needs and reproductive goals, ensuring a customized approach to the tissue freezing procedure.
  • We offer continuous support and guidance before, during, and after the tissue freezing procedure, ensuring a seamless experience.
  • Our commitment to excellence and patient-centered care makes us the preferred choice for tissue freezing in Ahmedabad.


How long can tissue be preserved?

Tissue can be preserved for extended periods, and in some cases, indefinitely. The duration depends on the quality of preservation and the method used.

Is tissue freezing safe?

When performed by experienced medical professionals using advanced cryopreservation techniques, tissue freezing is considered safe.

What is the success rate of tissue freezing?

Success rates vary based on the type of biological material and the expertise of the medical team, but tissue freezing has a high success rate when conducted by skilled professionals.

What is the cost of tissue freezing?

The costs of tissue freezing can vary based on the type of biological material being preserved and the services required.

Can anyone undergo tissue freezing?

Tissue freezing is available to individuals seeking fertility preservation, medical treatments, or research opportunities. A consultation with a medical professional is required to assess eligibility.

What is the difference between tissue freezing and traditional freezing?

Tissue freezing, or cryopreservation, is a specialized process that involves rapid cooling to extremely low temperatures to preserve biological materials like embryos, eggs, or sperm. Traditional freezing methods do not use such advanced technology and may not preserve biological materials as effectively.

How long can the preserved biological material last?

Biological material can be preserved indefinitely if stored correctly in ultra-low temperature tanks. Some centers have preserved material for several decades with high viability rates.

Is tissue freezing painful?

The tissue freezing procedure itself is not painful, as it generally involves collecting biological material in a clinical setting under sedation or anesthesia. Any discomfort typically arises during the initial collection or preparation phase, similar to other medical procedures.

Can I choose to thaw and use the preserved material whenever I want?

Yes, the preserved biological material can be thawed and used at a later date according to your needs and medical recommendations. This provides flexibility in scheduling treatments or medical interventions.

Can tissue freezing be performed on children or teenagers?

Tissue freezing is generally recommended for adults. However, in special circumstances, such as fertility preservation for medical treatments, consultations with healthcare professionals will determine eligibility.

How does tissue freezing impact future fertility?

Tissue freezing can preserve fertility for individuals who face medical treatments that might impact reproductive organs. The procedure has a high success rate when performed by experienced medical professionals.

Can same-sex couples or LGBTQ+ individuals benefit from tissue freezing?

Yes, tissue freezing offers reproductive options for individuals and couples of all orientations and identities. Advanced cryopreservation techniques can be used to preserve fertility and reproductive options for everyone.

Are there age limits for tissue freezing?

Age can play a role in the preservation of biological materials. Some fertility clinics may have guidelines or recommendations regarding the most suitable age range for tissue freezing to maximize the chances of successful outcomes.

Is tissue freezing only for fertility preservation?

No, tissue freezing can also be beneficial for medical research and regenerative medicine. It serves various purposes, including research, tissue banking, and specific treatments.

Can tissue freezing be used in conjunction with other fertility treatments?

Yes, tissue freezing can be a part of comprehensive fertility treatments. It can enhance the success rate of procedures such as in vitro fertilization (IVF) and assist with family planning.

Can tissue freezing be used for preserving other body tissues, like organs?

While tissue freezing primarily focuses on reproductive materials, research and advancements in cryopreservation techniques continue to expand, and there is potential for preserving other body tissues in the future.